It is used in quantitative PCR because the fluorescence can be measured at the end of each amplification cycle to determine, relatively or absolutely, how much DNA has been amplified. Quantitative PCR Master mix includes ROX, a passive reference dye; V = varies, different master mix versions are available with different amounts of ROX dye, or ROX included in a separate tube. The master mixes contain buffer, dNTPs, thermostable hot-start DNA polymerase, and of course, SYBR Green dye—everything needed for real-time PCR, except the sample and PCR primer pair. A basic mix, and the only one available without dUTP. Although there are now many choices of SYBR Green master mixes available, below are the recommended master mixes, by application.

Applied Biosystems™ SYBR™ Green master mixes are designed for quantitative real-time PCR using a set of two PCR primers that flank the target region. Applied Biosystems™ SYBR™ Green master mixes are designed for quantitative real-time PCR using a set of two PCR primers that flank the target region. All Rights Reserved. See the entire range of SYBR Green master mixes available from Thermo Fisher Scientific below. An updated, brighter version of Platinum SYBR Green supermix. Our brightest mix. For Research Use Only. For speed and efficiency, both LuminoCt® and KiCqStart® product lines are designed for Fast qPCR; the JumpStart™ products provide equally quality reagents for conventional qPCR. All of our SYBR® Green products are formulated with a hot-start mechanism for enhanced accuracy, precision, and sensitivity. JumpStart Taq ReadyMix for Conventional qPCR, Custom & Predesigned DNA Oligos & qPCR Probes, Advanced Genomics – CRISPR Technology & RNAi, Nucleic Acid Electrophoresis & Hybridization, Molecular Biology Newsletter Subscription, • For Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems, KiCqStart SYBR Green qPCR ReadyMix Low ROX™, KiCqStart SYBR Green qPCR ReadyMix with ROX™, KiCqStart SYBR Green qPCR ReadyMix for iQ™, SYBR Green JumpStart Taq ReadyMix™ for High Throughput QPCR, • Prepared with internal reference dye for high-throughput qPCR, SYBR Green JumpStart Taq ReadyMix for qPCR, SYBR Green JumpStart Taq ReadyMix without MgCl, SYBR Green JumpStart Taq ReadyMix for qPCR, Capillary Formulation. SYBR® Green I is a commonly used fluorescent dye that binds double-stranded DNA molecules by intercalating between the DNA bases. Includes dye in mix and in sample buffer to aid pipetting. > KiCqStart products are designed with a stringent hot-start mechanism for greater specificity. It is produced as a highly optimized ReadyMix developed with JumpStart Taq antibody for rapid activation and unparalleled sensitivity. SYBR® Green Based qPCR.

The ReadyMix virtually eliminates the need to optimize assay conditions and is compatible with commonly available qPCR instruments.

Linearity of pBAC-2cp 10-fold dilutions with SYBR Green JumpStart Taq ReadyMix, Figure 2. • = master mix includes uracil N-glycosylase (UNG); •• = master mix includes a heat-labile form of UNG, which improves master mix performance compared to the standard UNG.

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